pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
(Plasmid
#89587)
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PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
- Backbone size w/o insert (bp) 9530
- Total vector size (bp) 10266
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenuclear localization signal (nls) turboRFP
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Alt namenlsturboRFP
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SpeciesSV40, Entacmaea quadricolor
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Insert Size (bp)736
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer EBV_rev_primer (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Backbone plasmid pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) also submitted in addgene database.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE was a gift from Takuji Iwasato (Addgene plasmid # 89587 ; http://n2t.net/addgene:89587 ; RRID:Addgene_89587) -
For your References section:
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045
