pmCherry-MYRIP
(Plasmid #89586)

• Purpose: Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.
• Depositing Lab: Thomas Friedman
• Publication: Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583.

Backbone

• Vector backbone: pmCherry
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7281
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYRIP
• Species: M. musculus (mouse)
• Insert Size (bp): 2571
• GenBank ID: BC129851 (identical) NM_144557 (identical except for E593K)
• Entrez Gene: Myrip (a.k.a. A230081N12Rik, Slac, Slac2-c, Slac2c)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV_fwd_primer
• 3′ sequencing primer: EBV_rev_primer

Resource Information

• Supplemental Documents:
  • pmCherry-myRIP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry