pEGFP-MYO7A-TAIL
(Plasmid #89585)

• Purpose: Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.
• Depositing Lab: Thomas Friedman
• Publication: Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-08-0583.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 8625
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYO7A
• Species: M. musculus (mouse)
• Insert Size (bp): 3894
• Mutation: Truncation encoding amino acids 870-2166 of MYO7A (AAV87212.1)
• GenBank ID: AAV87212.1 AY821853
• Entrez Gene: Myo7a (a.k.a. Hdb, Myo7, USH1B, nmf371, polka, sh-1, sh1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP_C_primer
• 3′ sequencing primer: EBV_rev_primer

Resource Information

• Supplemental Documents:
  • pEGFP-MYO7A-TAIL.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry