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Addgene

pZa-tetO-csgB-CmR
(Plasmid #89580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZa
  • Backbone size w/o insert (bp) 2273
  • Total vector size (bp) 2729
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    csgB
  • Alt name
    minor curli subunit
  • Species
    E.coli
  • Insert Size (bp)
    456
  • GenBank ID
    NC_000913.3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer CCGGGTACCATGAAAAACAAATTGTTATTTATGAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZa-tetO-csgB-CmR was a gift from Urartu Seker (Addgene plasmid # 89580 ; http://n2t.net/addgene:89580 ; RRID:Addgene_89580)

  • For your References section:

    Genetically-Tunable Mechanical Properties of Bacterial Functional Amyloid Nanofibers. Abdelwahab MT, Kalyoncu E, Onur T, Baykara MZ, Seker UO. Langmuir. 2017 Apr 7. doi: 10.1021/acs.langmuir.7b00112. 10.1021/acs.langmuir.7b00112 PubMed 28388843
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