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Addgene

pEF6 rglut1 HA complete
(Plasmid #89572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF6
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pEF6 rglut1 HA complete
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Slc2a1 (a.k.a. GLUTB, GTG1, Glut1, Gtg3, RATGTG1)
  • Promoter EF-1α
  • Tags / Fusion Proteins
    • rglut1 HA complete (N terminal on insert)
    • V5-His A, B, C (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6 rglut1 HA complete was a gift from Jeffrey Rathmell (Addgene plasmid # 89572 ; http://n2t.net/addgene:89572 ; RRID:Addgene_89572)

  • For your References section:

    Cytokine stimulation promotes glucose uptake via phosphatidylinositol-3 kinase/Akt regulation of Glut1 activity and trafficking. Wieman HL, Wofford JA, Rathmell JC. Mol Biol Cell. 2007 Apr;18(4):1437-46. Epub 2007 Feb 14. 10.1091/mbc.e06-07-0593 PubMed 17301289
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