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Rc/CMV cyclin D2 LxCxE HA
(Plasmid #8957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    Deletion of LxCxE motif
  • Entrez Gene
    CCND2 (a.k.a. KIAK0002, MPPH3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by digesting the cyclin D2 cDNA in Bluescript SK+ with NcoI and RsrII to release a 34 bp fragment containg the LxCxE codons. This was replaced with a doublestranded oligo (5' CATGGAGGTGGACCCG3') that exactly lacks the LxCxE codons. For parent plasmid information, see Author's Map link for Rc/CMV cyclin D2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D2 LxCxE HA was a gift from Philip Hinds (Addgene plasmid # 8957 ; http://n2t.net/addgene:8957 ; RRID:Addgene_8957)

  • For your References section:

    Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Baker GL, Landis MW, Hinds PW. Cell Cycle. 2005 Feb . 4(2):330-8. 10.4161/cc.4.2.1485 PubMed 15684604
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