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Addgene

Rc/CMV cyclin D2 E7K HA
(Plasmid #8955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV
  • Backbone size w/o insert (bp) 5542
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin D2
  • Alt name
    cyclin D2 K7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    E7K: point mutation in LxCxE motif, LxCxk
  • Entrez Gene
    CCND2 (a.k.a. KIAK0002, MPPH3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    For parent plasmid information, see Author's Map link for Rc/CMV cyclin D2.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutant PCR product was digested with HindIII and RsrII and was used to replace the corresponding wild-type sequence of cyclin D2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rc/CMV cyclin D2 E7K HA was a gift from Philip Hinds (Addgene plasmid # 8955 ; http://n2t.net/addgene:8955 ; RRID:Addgene_8955)

  • For your References section:

    Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Baker GL, Landis MW, Hinds PW. Cell Cycle. 2005 Feb . 4(2):330-8. 10.4161/cc.4.2.1485 PubMed 15684604
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