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CeNL-Clathrin_pEGFP
(Plasmid #89540)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89540 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CeNL
  • Species
    Synthetic
  • Insert Size (bp)
    1200
  • GenBank ID
    LC128714
  • Promoter CMV
  • Tag / Fusion Protein
    • Clathrin light chain (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from NanoLuc luciferase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CeNL-Clathrin_pEGFP was a gift from Takeharu Nagai (Addgene plasmid # 89540 ; http://n2t.net/addgene:89540 ; RRID:Addgene_89540)

  • For your References section:

    Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T. Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718. 10.1038/ncomms13718 PubMed 27966527
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