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Addgene

pLenti-PRSX8ChR2 (H134R)eYFP
(Plasmid #89539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 9700
  • Modifications to backbone
    insertion of PRSX8 promoter
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChR2 (H134R)
  • Species
    Synthetic
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    modified from pLenti-CaMKIIa-hChR2(H134R)-eYFP from Karl Deisseroth
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PRSX8ChR2 (H134R)eYFP was a gift from Ruth Stornetta (Addgene plasmid # 89539 ; http://n2t.net/addgene:89539 ; RRID:Addgene_89539)

  • For your References section:

    The retrotrapezoid nucleus stimulates breathing by releasing glutamate in adult conscious mice. Holloway BB, Viar KE, Stornetta RL, Guyenet PG. Eur J Neurosci. 2015 Sep;42(6):2271-82. doi: 10.1111/ejn.12996. Epub 2015 Jul 23. 10.1111/ejn.12996 PubMed 26096172
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