Rc/CMV cyclin D2 K112E HA
(Plasmid
#8953)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRc/CMV
- Backbone size w/o insert (bp) 5542
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyclin D2
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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MutationK112E: point mutation in cyclin box
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Entrez GeneCCND2 (a.k.a. KIAK0002, MPPH3)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For parent plasmid information, see Author's Map link for Rc/CMV cyclin D2.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Rc/CMV cyclin D2 K112E HA was a gift from Philip Hinds (Addgene plasmid # 8953 ; http://n2t.net/addgene:8953 ; RRID:Addgene_8953) -
For your References section:
Multiple functions of D-type cyclins can antagonize pRb-mediated suppression of proliferation. Baker GL, Landis MW, Hinds PW. Cell Cycle. 2005 Feb . 4(2):330-8. 10.4161/cc.4.2.1485 PubMed 15684604