Rc/CMV cyclin D2 HA
(Plasmid #8950)

• Depositing Lab: Philip Hinds
• Publication: Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Backbone

• Vector backbone: pRc/CMV
• Backbone size w/o insert (bp): 5542
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D2
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: CCND2 (a.k.a. KIAK0002, MPPH3)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wildtype EcoRV to MluI fragment of cyclin D2 was replaced with a double stranded oligonucleotide. This resulted in a change at the C-terminus of cyclin D2 from DL to DLGYPYDVPDYADL and the deletion of 30 base pairs of 3' UTS between the stop codon and the MluI site. For parent plasmid information, see Author's Map link for Rc/CMV cyclin D2.