Tet-pLKO-FOXM1-shRNA-84
(Plasmid #89496)

• Purpose: Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
• Depositing Lab: Adam Karpf
• Publication: FOXM1 toolbox (unpublished)

Backbone

• Vector backbone: Tet-pLKO-puro
• Backbone size w/o insert (bp): 10633
• Total vector size (bp): 8816
• Vector type: Mammalian Expression, Lentiviral, RNAi ; Doxycycline inducible
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FOXM1
• gRNA/shRNA sequence: TTGCAGGGTGGTCCGTGTAAA
• Species: H. sapiens (human)
• Entrez Gene: FOXM1 (a.k.a. FKHL16, FOXM1A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT)
• Promoter: H1/TO

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: GGCAGGGATATTCACCATTATCGTTTCAGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FOXM1 shRNA (TRCN0000273984) sequence was retrieved from the Broad Genetic Perturbation Platform Web Portal. Oligos corresponding to the shRNA were annealed and ligated into Tet-pLKO-puro.