MSCV p2GM AMPK alpha2hp1 alpha1hp1
(Plasmid #89492)

• Purpose: shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression
• Depositing Lab: Russell Jones
• Publication: Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27.

Backbone

• Vector backbone: MSCV/LTRmiR30-PIGdeltaR1 (MSCV p2Gm)
• Backbone size w/o insert (bp): 7894
• Total vector size (bp): 8126
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 2 hairpins against mouse/human AMPKalpha1 and alpha2
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 232
• Promoter: U6 (The GFP-Puro is from a PGK promoter; the shRNA is from a U6 promoter)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GFP miR primer (GCTGGAGTTCGTGACCGCC)

Resource Information

• Supplemental Documents:
  • plasmid notes
  • Map and Sequence
  • Vector backbone
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry