LMP FFB
(Plasmid #89491)

• Purpose: Firefly hairpin
• Depositing Lab: Russell Jones
• Publication: Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27.

Backbone

• Vector backbone: LMP
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 7900
• Vector type: Mammalian Expression, Mouse Targeting, Retroviral
• Selectable markers: Puromycin ; GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Firefly luciferase hairpin, clone B
• Promoter: U6 (The GFP-Puro is from a PGK promoter; the shRNA is from a U6 promoter)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LMPseq1 or LMPseq2

Resource Information

• Supplemental Documents:
  • Map and Sequence
• A portion of this plasmid was derived from a plasmid made by: Ross Dickins in the Lowe lab, who also sold it to Open Biosystems
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

hairpin sequence 5'-AGCTCCCGTGAATTGGAATCC-3'