LMP AMPK alpha2 hp1
(Plasmid #89490)

• Purpose: shRNA against AMPK alpha2 in mouse/human to knock down protein expression
• Depositing Lab: Russell Jones
• Publication: Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27.

Backbone

• Vector backbone: LMP
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 8016
• Vector type: Mammalian Expression, Mouse Targeting, Retroviral
• Selectable markers: Puromycin ; GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hairpin against mouse/human AMPKalpha2
• Insert Size (bp): 116
• Entrez Gene: PRKAA2 (a.k.a. AMPK, AMPK2, AMPKa2, PRKAA)
• Entrez Gene: Prkaa2 (a.k.a. 2310008I11Rik, A830082D05, AMPKalpha2)
• Promoter: LMP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LMPseq1 or LMPseq2

Resource Information

• Supplemental Documents:
  • Map and Sequence
• A portion of this plasmid was derived from a plasmid made by: Ross Dickins in the Lowe lab, who also sold it to Open Biosystems
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry