LMP AMPK alpha1 hp1
(Plasmid #89489)

• Purpose: shRNA against AMPK alpha1 in mouse/human to knock down protein expression
• Depositing Lab: Russell Jones
• Publication: Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi: 10.1016/j.cmet.2012.12.001. Epub 2012 Dec 27.

Backbone

• Vector backbone: LMP
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 8013
• Modifications to backbone: None
• Vector type: Mammalian Expression, Mouse Targeting, Retroviral
• Selectable markers: Puromycin ; GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hairpin against mouse/human AMPKalpha1
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 19
• Entrez Gene: PRKAA1 (a.k.a. AMPK, AMPK alpha 1, AMPKa1)
• Entrez Gene: Prkaa1 (a.k.a. AMPKalpha1, C130083N04Rik)
• Promoter: U6 (The GFP-Puro is from a PGK promoter; the shRNA is from a U6 promoter)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LMPseq1 or LMPseq2

Resource Information

• Supplemental Documents:
  • Map and Sequence
• A portion of this plasmid was derived from a plasmid made by: Ross Dickins in the Lowe lab, who also sold it to Open Biosystems
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry