YFN43
(Plasmid #89487)

• Purpose: (Empty Backbone) gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP
• Depositing Lab: Alejandro Ferrando
• Publication: Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.1371/journal.pone.0046907. Epub 2012 Oct 8.

Backbone

• Vector backbone: pMDC43
• Vector type: Plant Expression
• Promoter: 2x35S
• Selectable markers: Hygromycin
• Tag / Fusion Protein:
  • YFP-N (aa 1-154) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: These pMDC43 derived plasmids have genes of resistance not only to Kanamycin but also to Amikacin, while the genes of resistance to Kan in the pDONR don't have it. So this way the recombinant clones between pYFN43 or pYFC43 with pDONr can be differentiated.
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To enhance folding of the fusion proteins a flexible linker was introduced between the coding sequence of either fragment of YFP and the attR recombination site. For more information see: http://www.ibmcp.upv.es/FerrandoLabVectors/