pBHRSF184
(Plasmid #89482)

• Purpose: Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)
• Depositing Lab: Hideo Iwai
• Publication: Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/1873-3468.12640.

Backbone

• Vector backbone: pHYRSF53
• Backbone size w/o insert (bp): 4540
• Total vector size (bp): 5245
• Modifications to backbone:
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Asparaginyl endopeptidase from Oldenlandia affinis
• Alt name: OaAEP1
• Species: Synthetic
• Insert Size (bp): 1353
• Promoter: T7
• Tag / Fusion Protein:
  • Smt3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Supplemental Documents:
  • pBHRSF184.gb
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry