ARHGEF11(DHPH)-CRY2-mCherry
(Plasmid #89481)

• Purpose: Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11
• Depositing Lab: Xavier Trepat Guixer
• Publication: Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10.1038/ncomms14396.

Backbone

• Vector backbone: pmCherryN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3939
• Total vector size (bp): 7663
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ARHGEF11(DHPH) - CRY2(1-498) - mCherry
• Alt name: optoGEF-RhoA
• Alt name: PDZ-RhoGEF(DHPH)-CRY2(1-498)-mCherry
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Entrez Gene: CRY2 (a.k.a. HCRY2, PHLL2)
• Entrez Gene: ARHGEF11 (a.k.a. GTRAP48, PDZ-RHOGEF)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CRY2(1-498)-mCherry fusion comes from the lab of Chandra Tucker: pCRY2PHR-mCherryN1 (plasmid # 26866)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry