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PurposeEncodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3985
- Total vector size (bp) 5434
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemito-CIBN-GFP
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Entrez GeneCIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInitial vector was obtained from addgene, number:26867
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mito-CIBN-GFP was a gift from Xavier Trepat Guixer (Addgene plasmid # 89480 ; http://n2t.net/addgene:89480 ; RRID:Addgene_89480) -
For your References section:
Optogenetic control of cellular forces and mechanotransduction. Valon L, Marin-Llaurado A, Wyatt T, Charras G, Trepat X. Nat Commun. 2017 Feb 10;8:14396. doi: 10.1038/ncomms14396. 10.1038/ncomms14396 PubMed 28186127
