Rc/CMV cyclin D1 HA
(Plasmid #8948)

• Depositing Lab: Philip Hinds
• Publication: Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Backbone

• Vector backbone: pRc/CMV
• Backbone size w/o insert (bp): 5542
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin D1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Entrez Gene: CCND1 (a.k.a. BCL1, D11S287E, PRAD1, U21B31)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A PCR product was digested with StuI and BspEI and the resulting ~250 bp fragment was used to replace the corresponding wildtype fragment. This resulted in the addition of the HA epitope after the final amino acid of cyclin D1 and reiteration of the final two cyclind D1 residues (DI) after the tag. In addition, 80 bp of the 3' UTS is deleted. For parent plasmid information, see Author's Map link for Rc/CMV cyclin D1.