VN-alpha Synuclein
(Plasmid #89470)

• Purpose: expresses human alpha Synuclein fused to the N-terminal part of Venus
• Depositing Labs: Bradley Hyman, Tiago Outeiro
• Publication: Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10.1371/journal.pone.0001867.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5943
• Total vector size (bp): 6366
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNCA
• Species: H. sapiens (human)
• Insert Size (bp): 423
• GenBank ID: NM_000345
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal part of Venus (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry