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pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA
(Plasmid #89459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHD7
  • Alt name
    chromodomain helicase DNA binding protein 7
  • Alt name
    NC_00008
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9293
  • Entrez Gene
    CHD7 (a.k.a. CRG, HH5, IS3, KAL5)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI/XbaI (destroyed during cloning)
  • 3′ cloning site SwaI/SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA was a gift from Peter Scacheri (Addgene plasmid # 89459 ; http://n2t.net/addgene:89459 ; RRID:Addgene_89459)
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