pCIneo-hCHD7
(Plasmid #89458)

• Purpose: constitutive expression of human CHD7 in mammalian cells
• Depositing Lab: Peter Scacheri
• Publication: Peter Scacheri lab plasmids (unpublished)

Backbone

• Vector backbone: pCIneo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5470
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHD7
• Alt name: chromodomain helicase DNA binding protein 7
• Alt name: NC_00008
• Species: H. sapiens (human)
• Insert Size (bp): 9230
• Entrez Gene: CHD7 (a.k.a. CRG, HH5, IS3, KAL5)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI/XbaI (destroyed during cloning)
• 3′ cloning site: SwaI/SmaI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

includes th enative ATG context and native polyA adenylation signal. Note: polymorphic or introduced gga>ggg at Gly360. mutated cct>cca of Pro 481 to create PflMI site. Mutated ttc>ttt of Phe 1035 to create BstBI site