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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS/U6
- Backbone size w/o insert (bp) 3300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameezrin siRNA
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Alt nameezrin
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SpeciesH. sapiens (human)
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Insert Size (bp)50
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Entrez GeneEZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
siRNA targeting ezrin. Sense strand of the hairpin is identical to a 22 nucleotide region in the target gene (ezrin [376-396]). Sequence is GGGTC CTACG CTGTG CAGGC CAAG.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS/U6 ezrin siRNA was a gift from Philip Hinds (Addgene plasmid # 8945 ; http://n2t.net/addgene:8945 ; RRID:Addgene_8945) -
For your References section:
Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype. Yang HS, Hinds PW. Mol Cell. 2003 May . 11(5):1163-76. 10.1016/S1097-2765(03)00135-7 PubMed 12769842