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Addgene

pBS/U6 ezrin siRNA
(Plasmid #8945)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8945 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS/U6
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ezrin siRNA
  • Alt name
    ezrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    50
  • Entrez Gene
    EZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA targeting ezrin. Sense strand of the hairpin is identical to a 22 nucleotide region in the target gene (ezrin [376-396]). Sequence is GGGTC CTACG CTGTG CAGGC CAAG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS/U6 ezrin siRNA was a gift from Philip Hinds (Addgene plasmid # 8945 ; http://n2t.net/addgene:8945 ; RRID:Addgene_8945)
  • For your References section:

    Increased ezrin expression and activation by CDK5 coincident with acquisition of the senescent phenotype. Yang HS, Hinds PW. Mol Cell. 2003 May . 11(5):1163-76. 10.1016/S1097-2765(03)00135-7 PubMed 12769842