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Addgene

mCh-GluA1-CIB
(Plasmid #89444)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89444 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCaMKII
  • Backbone manufacturer
    custom
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCh-GluA1-CIB
  • Alt name
    GluR1
  • Species
    R. norvegicus (rat)
  • Promoter CaMKII

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

use ~500ng/well for 12 well plate

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-GluA1-CIB was a gift from Matthew Kennedy (Addgene plasmid # 89444 ; http://n2t.net/addgene:89444 ; RRID:Addgene_89444)
  • For your References section:

    Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. 10.1016/j.neuron.2016.12.037 PubMed 28132827