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PurposeAllows conditional recruitment of AMPA receptors to the postsynaptic membrane
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaMKII
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Backbone manufacturercustom
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCh-GluA1-CIB
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Alt nameGluR1
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SpeciesR. norvegicus (rat)
- Promoter CaMKII
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
use ~500ng/well for 12 well plate
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCh-GluA1-CIB was a gift from Matthew Kennedy (Addgene plasmid # 89444 ; http://n2t.net/addgene:89444 ; RRID:Addgene_89444) -
For your References section:
Optogenetic Control of Synaptic Composition and Function. Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, Dell'Acqua ML, Kennedy MJ. Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26. 10.1016/j.neuron.2016.12.037 PubMed 28132827