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Addgene

pEGFP-hSyntenin2b
(Plasmid #89437)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89437 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SDCBP2
  • Alt name
    Syntenin-2
  • Species
    H. sapiens (human)
  • Mutation
    *see below
  • Entrez Gene
    SDCBP2 (a.k.a. SITAC, SITAC18, ST-2, ST2)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control found a R138C mutation in the SDCBP2 insert. The affect of this mutation is not known.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hSyntenin2b was a gift from Fritz Rathjen (Addgene plasmid # 89437 ; http://n2t.net/addgene:89437 ; RRID:Addgene_89437)

  • For your References section:

    The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. Koroll M, Rathjen FG, Volkmer H. J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 10.1074/jbc.M010647200. Epub 2001 Jan 4. 10.1074/jbc.M010647200 PubMed 11152476
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