pSG5-hSyntenin2a
(Plasmid
#89432)
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Purposemammalian expression of human Syntenin2a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSDCBP2
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Alt nameSyntenin-2
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SpeciesH. sapiens (human)
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Entrez GeneSDCBP2 (a.k.a. SITAC, SITAC18, ST-2, ST2)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-hSyntenin2a was a gift from Fritz Rathjen (Addgene plasmid # 89432 ; http://n2t.net/addgene:89432 ; RRID:Addgene_89432) -
For your References section:
The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. Koroll M, Rathjen FG, Volkmer H. J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 10.1074/jbc.M010647200. Epub 2001 Jan 4. 10.1074/jbc.M010647200 PubMed 11152476