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Addgene

pPS2972
(Plasmid #8942)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NDR1
  • Alt name
    pMSCV/U6-NDR
  • Alt name
    siRNA NDR1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_007271
  • Entrez Gene
    STK38 (a.k.a. NDR, NDR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA targeting NDR1. BamHI-BamHI fragment from pBS/U6-NDR blunt-ended and inserted into pMSCVpuro blunt ended at the unique NsiI site. Small hairpin RNA corresponds to nucleotides 515-535 of NDR ORF (nucleotides 801-821 of NDR mRNA sequence, Genbank NM_007271).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2972 was a gift from Pamela Silver (Addgene plasmid # 8942 ; http://n2t.net/addgene:8942 ; RRID:Addgene_8942)

  • For your References section:

    Retrovirus-delivered siRNA. Devroe E, Silver PA. BMC Biotechnol. 2002 Aug 28. 2():15. 10.1186/1472-6750-2-15 PubMed 12199908
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