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Addgene

pGBT9-chNfasc (+RSLE)
(Plasmid #89418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT9
  • Backbone manufacturer
    Clontech
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFASC
  • Alt name
    Neurofascin
  • Species
    G. gallus (chicken)
  • Entrez Gene
    NFASC
  • Promoter ADH1
  • Tag / Fusion Protein
    • GAL4 DBD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PstI (unknown if destroyed)
  • 5′ sequencing primer Gal4-N-term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: This plasmid contains amino acids 1160 - 1272 of the NFASC insert (NP_001004493.1).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT9-chNfasc (+RSLE) was a gift from Fritz Rathjen (Addgene plasmid # 89418 ; http://n2t.net/addgene:89418 ; RRID:Addgene_89418)

  • For your References section:

    The neural cell recognition molecule neurofascin interacts with syntenin-1 but not with syntenin-2, both of which reveal self-associating activity. Koroll M, Rathjen FG, Volkmer H. J Biol Chem. 2001 Apr 6;276(14):10646-54. doi: 10.1074/jbc.M010647200. Epub 2001 Jan 4. 10.1074/jbc.M010647200 PubMed 11152476

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