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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCAF
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Alt nameP/CAF
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SpeciesH. sapiens (human)
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Insert Size (bp)2579
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GenBank IDNM_003884
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Entrez GeneKAT2B (a.k.a. CAF, P/CAF, PCAF)
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Tag
/ Fusion Protein
- flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Compared to GenBank reference sequence NP_003875.3, Serine-2 is actually Valine-2 immediately after the FLAG tag.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI flag PCAF was a gift from Yoshihiro Nakatani (Addgene plasmid # 8941 ; http://n2t.net/addgene:8941 ; RRID:Addgene_8941) -
For your References section:
A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Yang XJ, Ogryzko VV, Nishikawa J, Howard BH, Nakatani Y. Nature. 1996 Jul 25. 382(6589):319-24. 10.1038/382319a0 PubMed 8684459
Map uploaded by the depositor.
