crestin
(Plasmid
#89394)
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PurposeFor in situ hybridization, linearize with Sac I use T7 polymerase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonebluescript
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Vector typein situ probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecrestin
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3900
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Entrez Genecrestin
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make antisense RNA probe for in situ hybridization cut with Sac I and use T7 polymerase
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
crestin was a gift from Marnie Halpern (Addgene plasmid # 89394 ; http://n2t.net/addgene:89394 ; RRID:Addgene_89394) -
For your References section:
Genes dependent on zebrafish cyclops function identified by AFLP differential gene expression screen. Rubinstein AL, Lee D, Luo R, Henion PD, Halpern ME. Genesis. 2000 Jan;26(1):86-97. 10.1002/(SICI)1526-968X(200001)26:1<86::AID-GENE11>3.0.CO;2-Q PubMed 10660676