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Addgene

crestin
(Plasmid #89394)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89394 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    bluescript
  • Vector type
    in situ probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    crestin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3900
  • Entrez Gene
    crestin

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make antisense RNA probe for in situ hybridization cut with Sac I and use T7 polymerase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    crestin was a gift from Marnie Halpern (Addgene plasmid # 89394 ; http://n2t.net/addgene:89394 ; RRID:Addgene_89394)
  • For your References section:

    Genes dependent on zebrafish cyclops function identified by AFLP differential gene expression screen. Rubinstein AL, Lee D, Luo R, Henion PD, Halpern ME. Genesis. 2000 Jan;26(1):86-97. 10.1002/(SICI)1526-968X(200001)26:1<86::AID-GENE11>3.0.CO;2-Q PubMed 10660676