pFS346
(Plasmid
#89350)
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PurposeRenilla Luciferase C-term tagging vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFS275
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Backbone manufacturerSato et al., Yeast, 22, 583-591, 2005
- Backbone size w/o insert (bp) 2734
- Total vector size (bp) 4866
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Modifications to backboneRluc replaced GFP in pFS275
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Vector typeC-terminal tagging vector
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Selectable markersnourseothricin resistance
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla luciferase
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SpeciesRenilla reniformis
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Insert Size (bp)2132
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer GCATTAATTAACACTTCGAAAGTTTATGATCCAG
- 3′ sequencing primer GCAGGCGCGCCCTATTGTTCATTTTTGAGAACTCGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFS346 was a gift from Nick Rhind (Addgene plasmid # 89350 ; http://n2t.net/addgene:89350 ; RRID:Addgene_89350) -
For your References section:
Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast. Keifenheim D, Sun XM, D'Souza E, Ohira MJ, Magner M, Mayhew MB, Marguerat S, Rhind N. Curr Biol. 2017 May 22;27(10):1491-1497.e4. doi: 10.1016/j.cub.2017.04.016. Epub 2017 May 4. 10.1016/j.cub.2017.04.016 PubMed 28479325