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Addgene

pFS345
(Plasmid #89349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-KanMX6
  • Backbone manufacturer
    https://www.addgene.org/39292/
  • Backbone size w/o insert (bp) 4882
  • Total vector size (bp) 5818
  • Vector type
    C-terminal tagging vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beetle luciferase
  • Species
    Photinus pyralis
  • Insert Size (bp)
    3086

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer GCATTAATTAACGAAGACGCCAAAAACATAAAGA
  • 3′ sequencing primer GCAGGCGCGCCCTACACGGCGATCTTTCCGCCCTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFS345 was a gift from Nick Rhind (Addgene plasmid # 89349 ; http://n2t.net/addgene:89349 ; RRID:Addgene_89349)

  • For your References section:

    Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast. Keifenheim D, Sun XM, D'Souza E, Ohira MJ, Magner M, Mayhew MB, Marguerat S, Rhind N. Curr Biol. 2017 May 22;27(10):1491-1497.e4. doi: 10.1016/j.cub.2017.04.016. Epub 2017 May 4. 10.1016/j.cub.2017.04.016 PubMed 28479325
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