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Addgene

pET-sfGFP-GGS9-Cre-6xHis
(Plasmid #89343)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89343 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET29
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5073
  • Total vector size (bp) 7003
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can also maintain in Mach1. For protein expression, transform into BL21 Star (DE3) cells. Induce at 0.6 OD with 0.5 mM IPTG final concentration. Growing overnight (16 hours) at 20-25 C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sf-GFP-GGS9-Cre-6xHis
  • Species
    Synthetic
  • Insert Size (bp)
    1930
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-sfGFP-GGS9-Cre-6xHis was a gift from David Liu (Addgene plasmid # 89343 ; http://n2t.net/addgene:89343 ; RRID:Addgene_89343)