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Addgene

pcDNAZeo(-) LAMP2C
(Plasmid #89342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/Zeo(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6236
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lysosomal-associated membrane protein 2C
  • Alt name
    LAMP-2C
  • Alt name
    LAMP2C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • GenBank ID
    AY561849.1
  • Entrez Gene
    LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAZeo(-) LAMP2C was a gift from Janice Blum (Addgene plasmid # 89342 ; http://n2t.net/addgene:89342 ; RRID:Addgene_89342)

  • For your References section:

    LAMP-2C Inhibits MHC Class II Presentation of Cytoplasmic Antigens by Disrupting Chaperone-Mediated Autophagy. Perez L, McLetchie S, Gardiner GJ, Deffit SN, Zhou D, Blum JS. J Immunol. 2016 Mar 15;196(6):2457-65. doi: 10.4049/jimmunol.1501476. Epub 2016 Feb 8. 10.4049/jimmunol.1501476 PubMed 26856698
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