pG5ElbLuc
(Plasmid
#89341)
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Purpose(Empty Backbone) The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepG5ElbLuc
- Backbone size (bp) 4955
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Modifications to backboneThe plasmid pG5ElbLuc was prepared from pG5ElbCAT (Lillie and Green, 1989) by removing the CAT gene by restriction digestion (EcoRI and Styl) and inserting the firefly luciferase gene (from pGEM-Luc) as a blunt-ended 1745-bp StuI-Hind111 fragment.
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG5ElbLuc was a gift from Roger Davis (Addgene plasmid # 89341 ; http://n2t.net/addgene:89341 ; RRID:Addgene_89341) -
For your References section:
Signal transduction within the nucleus by mitogen-activated protein kinase. Seth A, Gonzalez FA, Gupta S, Raden DL, Davis RJ. J Biol Chem. 1992 Dec 5;267(34):24796-804. PubMed 1332967
