pSG424 ELKC(S383A)
(Plasmid
#89334)
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PurposeElk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG424
- Backbone size w/o insert (bp) 3396
- Total vector size (bp) 3517
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameELK1
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Alt nameELK1, ETS transcription factor
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SpeciesH. sapiens (human)
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Insert Size (bp)365
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Mutationencodes aa 307-428, changed serine 383 to alanine 383
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GenBank IDNC_000023.11
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Entrez GeneELK1
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Tag
/ Fusion Protein
- Gal 4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer SV40pro_F_primer
- 3′ sequencing primer pBRev Bam_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byR. Marais, J. Wynne, R. Treisman, Cell 73, 381
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG424 ELKC(S383A) was a gift from Roger Davis (Addgene plasmid # 89334 ; http://n2t.net/addgene:89334 ; RRID:Addgene_89334) -
For your References section:
Integration of MAP kinase signal transduction pathways at the serum response element. Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ. Science. 1995 Jul 21. 269(5222):403-7. 10.1126/science.7618106 PubMed 7618106