pSG424 ELKC(S383A)
(Plasmid #89334)

• Purpose: Elk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1
• Depositing Lab: Roger Davis
• Publication: Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Backbone

• Vector backbone: pSG424
• Backbone size w/o insert (bp): 3396
• Total vector size (bp): 3517
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELK1
• Alt name: ELK1, ETS transcription factor
• Species: H. sapiens (human)
• Insert Size (bp): 365
• Mutation: encodes aa 307-428, changed serine 383 to alanine 383
• GenBank ID: NC_000023.11
• Entrez Gene: ELK1
• Tag / Fusion Protein:
  • Gal 4 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: SV40pro_F_primer
• 3′ sequencing primer: pBRev Bam_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: R. Marais, J. Wynne, R. Treisman, Cell 73, 381

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry