PTEN-VC
(Plasmid #89332)

• Purpose: expresses hPTEN fused to the C-terminal part of Venus
• Depositing Lab: Tiago Outeiro
• Publication: Unpublished plasmid (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5642
• Total vector size (bp): 7155
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PTEN1
• Species: H. sapiens (human)
• Insert Size (bp): 1212
• GenBank ID: NM_000314 NM_000314
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • C-terminal part of Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone (alpha-Synuclein-VC) comes from Pamela J.McLean.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original clone (alpha Synuclein-VC) includes already the C-terminal part of venus, and was a gift from Pamela J.McLean.