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pPS835
(Plasmid #8933)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPS293
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GSP1
  • Alt name
    YepGAL GSP1 G21V myc
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    G21V, stabilizes the GTP-bound form
  • Entrez Gene
    GSP1 (a.k.a. YLR293C, CNR1, CST17)
  • Tag / Fusion Protein
    • myc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gal-driven GSP1 G21V, myc tagged, 2 micron origin. Compare with pPS833.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS835 was a gift from Pamela Silver (Addgene plasmid # 8933 ; http://n2t.net/addgene:8933 ; RRID:Addgene_8933)

  • For your References section:

    The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Schlenstedt G, Saavedra C, Loeb JD, Cole CN, Silver PA. Proc Natl Acad Sci U S A 1995 Jan 3;92(1):225-9. 10.1073/pnas.92.1.225 PubMed 7816822
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