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pTRE/SP M1C448Y
(Plasmid #89326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRE-Tight
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spastin
  • Alt name
    SPAST
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1929
  • Mutation
    1-194 bp of 5'UTR deleted, C448Y
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter Tet-responsive P tight

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site BamH I (destroyed during cloning)
  • 5′ sequencing primer LNCX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin. To detect spastin use an anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE/SP M1C448Y was a gift from Peter Baas (Addgene plasmid # 89326 ; http://n2t.net/addgene:89326 ; RRID:Addgene_89326)

  • For your References section:

    Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics. Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW. J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 10.1523/JNEUROSCI.3309-13.2014. 10.1523/JNEUROSCI.3309-13.2014 PubMed 24478365
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