GFP/WT SP M85
(Plasmid
#89317)
-
PurposeExpression of mouse GFP/WT spastin M85 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 89317 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP C-1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSPAST M85
-
Alt nameBC046282
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1590
-
Entrez GeneSpast (a.k.a. Spg4, mKIAA1083)
- Promoter CMV
-
Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
GFP/WT SP M85 was a gift from Peter Baas (Addgene plasmid # 89317 ; http://n2t.net/addgene:89317 ; RRID:Addgene_89317) -
For your References section:
Quantitative and functional analyses of spastin in the nervous system: implications for hereditary spastic paraplegia. Solowska JM, Morfini G, Falnikar A, Himes BT, Brady ST, Huang D, Baas PW. J Neurosci. 2008 Feb 27;28(9):2147-57. doi: 10.1523/JNEUROSCI.3159-07.2008. 10.1523/JNEUROSCI.3159-07.2008 PubMed 18305248