GFP/KATNA1
(Plasmid #89316)

• Purpose: Expression of rat GFP/Katanin A subunit in mammalian cells
• Depositing Lab: Peter Baas
• Publication: Karabay et al J Neurosci. 2004 Jun 23;24(25):5778-88.

Backbone

• Vector backbone: pEGFP C-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KATNA1
• Alt name: katanin p60
• Alt name: AY621629
• Species: R. norvegicus (rat)
• Insert Size (bp): 1476
• Entrez Gene: Katna1
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry