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Addgene

pMT-HA-STX4
(Plasmid #89311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT/V5-HIS
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3538
  • Total vector size (bp) 4567
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STX4
  • Alt name
    syntaxin 4
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1092
  • Entrez Gene
    Syx4 (a.k.a. Dmel_CG2715, CG2715, Dm Syx4, DmSyx4, Dmel\CG2715, EG:95B7.1, EG:95B7.11, STX4, Syx, Syx-4, syx4)
  • Promoter P-MT (Metallothionein)
  • Tag / Fusion Protein
    • HA tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CATCTCAGTGCAACTAAA
  • 3′ sequencing primer CCTCGACTGTGCCTTCTA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-HA-STX4 was a gift from Andrew Peden (Addgene plasmid # 89311 ; http://n2t.net/addgene:89311 ; RRID:Addgene_89311)
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