pMT-HA-STX1
(Plasmid #89310)

• Purpose: Expresses HA tagged syntaxin 1 in Drosophila cells
• Depositing Lab: Andrew Peden
• Publication: Peden lab plasmids (unpublished)

Backbone

• Vector backbone: pMT/V5-HIS
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3538
• Total vector size (bp): 4441
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STX1
• Alt name: syntaxin 1
• Species: D. melanogaster (fly)
• Insert Size (bp): 903
• Entrez Gene: Syx1A (a.k.a. Dmel_CG31136, CG10716, CG18615, CG31136, CG5448, CT30033, Dm Syx1, DmSyx1A, Dmel\CG31136, STX1, SYX, SYX 1A, SYX1A, Stx1a, Sx1A, Syt1A, Syx, Syx-1A, Syx1, Syx1a, SyxA, anon-EST:Gibbs4, anon-WO02059370.54, dSyx1, dsyn-1A, l(3)06737, synt, syt-1A, sytx, syx, syx-1, syx-1A, syx1, syx1A)
• Promoter: P-MT (Metallothionein)
• Tag / Fusion Protein:
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CATCTCAGTGCAACTAAA
• 3′ sequencing primer: CCTCGACTGTGCCTTCTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry