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Addgene

pPS2991
(Plasmid #8931)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECMV-FLAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NDR2
  • Alt name
    pFLAG-NDR2
  • Species
    H. sapiens (human)
  • Entrez Gene
    STK38L (a.k.a. NDR2)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NDR2 ORF PCR amplified from Hela cDNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS2991 was a gift from Pamela Silver (Addgene plasmid # 8931 ; http://n2t.net/addgene:8931 ; RRID:Addgene_8931)

  • For your References section:

    Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. Devroe E, Erdjument-Bromage H, Tempst P, Silver PA. J Biol Chem. 2004 Jun 4. 279(23):24444-51. 10.1074/jbc.M401999200 PubMed 15067004
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