pBMN PLEKHM1-HA
(Plasmid
#89300)
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PurposeFor expression of PLEKHM1 tagged with HA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBMN
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLEKHM1
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Alt nameKIAA0356
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Alt nameAP162
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SpeciesH. sapiens (human)
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Entrez GenePLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBMN 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN PLEKHM1-HA was a gift from Michael Lazarou (Addgene plasmid # 89300 ; http://n2t.net/addgene:89300 ; RRID:Addgene_89300) -
For your References section:
Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M. J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18. 10.1083/jcb.201607039 PubMed 27864321