pPS2989
(Plasmid #8930)

• Depositing Lab: Pamela Silver
• Publication: Devroe et al J Cell Sci. 2003 Nov 1. 116(Pt 21):4401-8.

Backbone

• Vector backbone: pEGFP-PK
• Backbone manufacturer: Warner Greene
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IN
• Alt name: pEGFP-PK-IN
• Alt name: HIV-1 integrase
• Species: HIV
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • PK (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Generates alternate linker in between PK and IN to compare to results of pPS2986. Linker is derived from GFP-IN plasmids used in Limon 2002 paper--Pubmed ID 12368302) Localizes to cytoplasm.