pMX-IY LC3B
(Plasmid #89291)

• Purpose: For expression of the mammalian Atg8 protein LC3B without a tag
• Depositing Lab: Michael Lazarou
• Publication: Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18.

Backbone

• Vector backbone: pMX
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: IRES-YFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LC3B
• Alt name: MAP1LC3B
• Alt name: ATG8F
• Species: H. sapiens (human)
• Entrez Gene: MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
• Tag / Fusion Protein:
  • IRES-YFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBMN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry