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pET151-Vps4(101-437)-Hcp1 R325D
(Plasmid #89289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Vacuolar protein sorting-associated protein 4
  • Alt name
    Vps4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1554
  • Mutation
    deleted amino acids 1-100, mutated Arg-325 to Asp
  • GenBank ID
    NM_001184270
  • Entrez Gene
    VPS4 (a.k.a. YPR173C, CSC1, DID6, END13, GRD13, VPL4, VPT10)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis-V5-TEV (N terminal on backbone)
    • Hcp1 (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-Vps4(101-437)-Hcp1 R325D was a gift from Chris Hill (Addgene plasmid # 89289 ; http://n2t.net/addgene:89289 ; RRID:Addgene_89289)

  • For your References section:

    Structural Basis of Protein Translocation by the Vps4-Vta1 AAA ATPase. Monroe N, Han H, Shen PS, Sundquist WI, Hill CP. Elife. 2017 Apr 5;6. pii: e24487. doi: 10.7554/eLife.24487. 10.7554/eLife.24487 PubMed 28379137
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